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To assist organizational leaders and staff working in health and social service sectors to reduce stigma and successfully implement harm reduction interventions, we developed the 7 step harm reduction implementation framework (HRIF). The goal of the HRIF is to promote an organizational culture that supports harm reduction philosophy, policies and practices to enhance reach, scale up, and implementation of harm reduction interventions to reduce harms of substance use. Each of the seven steps in the framework includes a checklist, followed by a description of that step. This framework is focused on creating an organizational culture and the necessary conditions for effective implementation of harm reduction initiatives.